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Nucleotide sequence and expression of nif HD from Frankia strain EuIKl, a symbiont of Elaeagnus umbellata
Author(s) -
Kim Ho Bang,
An Chung Sun
Publication year - 1997
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1997.tb05373.x
Subject(s) - frankia , biology , nucleic acid sequence , microbiology and biotechnology , clone (java method) , genetics , sequence analysis , open reading frame , peptide sequence , gene , root nodule , bacteria , symbiosis
Nucleotide sequences of nif HD from Frankia strain EuIKl were determined and analysed. The 3.2‐kb and 5.5‐kb Bam HI fragments of a genomic clone were previously shown to contain nif HD‐like sequences and to be contiguous, based on hybridization experiments and partial sequencing data. Sequence analysis of about 3.0 kb from these fragments revealed two open reading frames and beginning of a third in the same orientation, each of which showed high degree of sequence homology with nif H, nif D and nif K, respectively. The deduced amino acid sequence of the nif H ORF, consisting of 861 bp, showed sequence similarity of about 91% with those of other Frankia strains, whereas that of nif D ORF, consisting of 1458 bp, showed about 85% similarity. Intergenic sequence between nif H and nif D was 45 bp and between nif D and nif K was 61 bp. The 5’of nif H revealed putative Shine‐Dalgarno sequences; however, a sequence resembling a typical nif ‐promoter or NifA binding site was not found. Northern hybridization of RNA from the nodules showed that nif HDK were transcribed into a polycistronic mRNA in the symbiont of Elaeagnus umbellata .