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Tissue specific modulation of salt inducible gene expression: Callus versus whole plant response in salt tolerant alfalfa
Author(s) -
Winicov Hga,
Shirzadegan Magid
Publication year - 1997
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1997.tb04788.x
Subject(s) - callus , gene , biology , gene expression , salt (chemistry) , medicago sativa , regulation of gene expression , messenger rna , microbiology and biotechnology , botany , genetics , chemistry
We have cloned several genes that were continuously induced by salt in our salt‐tolerant alfalfa ( Medicago sativa L .) callus. These genes were not induced by 24 h salt stress in the parent salt‐sensitive cell line. To investigate the extent to which salt‐stress regulation of these genes is influenced by a program of tissue specific regulation we have characterized another one of the cloned cDNAs, pA18 , determined the expression of the transcript it encodes and compared its salt‐inducible tissue‐specific expression in plants with MsPRP2 , another single or low copy gene induced in the salt‐tolerant cells. While pA18 and MsPRP2 transcripts are similarly salt‐induced in callus, comparisons of tissue specific expression between these two genes demonstrate that regulated tissue specific expression can override salt inducibility of mRNA accumulation for individual genes.