Premium
Tonoplast H + pumps are activated during callus formation of tuber tissues of Jerusalem artichoke ( Helianthus tuberosus )
Author(s) -
Ueoka Hanayo,
Hase Akira
Publication year - 1997
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1997.tb03458.x
Subject(s) - callus , jerusalem artichoke , helianthus , vacuole , chromosomal translocation , vesicle , biochemistry , atpase , chemistry , biology , botany , cytoplasm , horticulture , enzyme , membrane , sunflower , gene
Changes in tonoplast H + ‐ATPase (EC 3.6.1.3) and H + –PPase (EC 3.6.1.1) activities were examined during the early period of callus formation in tuber tissues of Jerusalem artichoke ( Helianthus tuberosus L.). In callus‐forming tissues cultured on a medium containing 2,4‐D, the ATP‐dependent H + ‐translocation activity of tonoplast vesicles increased 3‐fold after a 2‐day lag phase, while the ATP‐hydrolytic activity and amount of tonoplast H + ‐ATPase protein were relatively constant after the lag phase. In the control tissue disks cultured on a medium free of 2,4‐D, large declines in ATP‐hydrolytic and ATP‐dependent H + ‐translocation activities were observed. By contrast, the PP‐dependent H + ‐translocation activity of tonoplast vesicles increased about 8‐fold during the first 3 days of culture without any lag phase, and regardless of the presence of 2,4‐D in the culture medium. However, the PP‐hydrolytic activity and amount of H + ‐PPase protein did not change during the culture period, independently of callus formation. Transfer of the control tissue disks to the 2,4‐D‐containing medium, however, resulted in a further rapid stimulation of PP‐dependent H + ‐translocation as well as an activation of ATP‐dependent H + ‐translocation. These results suggest that both tonoplast H + pumps are involved in callus formation of tuber tissues of Jerusalem artichoke.