Premium
Distinct pathways for jasmonate‐ and elicitor‐induced expressions of a cytochrome P450 gene in soybean suspension‐cultured cells
Author(s) -
Ohta Hiroyuki,
Suzuki Genki,
Awai Koichiro,
Masuda Tatsuru,
Kato Tomohiko,
Shibata Daisuke,
Takamiya Kenichiro
Publication year - 1997
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1997.tb03071.x
Subject(s) - elicitor , methyl jasmonate , biology , jasmonate , gene expression , cytochrome p450 , trifluoperazine , biochemistry , microbiology and biotechnology , gene , calmodulin , arabidopsis , enzyme , mutant
The mechanisms of the jasmonate‐induced expression of genes encoding the cytochrome P450 CYP93A1 and lipoxygenase L‐4 were analyzed in a soybean photomixotrophic cultured cell line, SB‐P. The induction of the cytochrome P450 gene caused by methyl jasmonate (MeJA) was specifically suppressed by trifluoperazine and DCMU, inhibitors of chloroplast electron transport. Additionally, induction of the cytochrome P450 gene required irradiation. In contrast, induction of the lipoxygenase L‐4 gene by the MeJA treatment occurred in both dark and light. Based on the results, the presence of two distinct signalling pathways for jasmonate‐inducible gene expression, light‐dependent and light‐independent, is proposed. The jasmonate‐inducible cytochrome P450 was also specifically induced by a fungal elicitor from a cell wall fraction of Phytophthora megasperma , a fungal pathogen, suggesting a role for P450 in the defense response to fungus in soybean cells. However, trifluoperazine did not block the elicitor‐induced expression of cytochrome P450.