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Cloning, characterization and transcriptional studies of ferredoxin genes from the mesophilic cyanobacterium Synechocystis sp. PCC 6803 and the thermophilic cyanobacterium Synechococcus vulcanus
Author(s) -
Nakamoto Hitoshi,
Suzuki Toyoko
Publication year - 1997
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1997.tb01837.x
Subject(s) - cyanobacteria , ferredoxin , synechococcus , thermophile , synechocystis , biology , southern blot , gene , cloning (programming) , northern blot , biochemistry , rna , bacteria , genetics , enzyme , computer science , programming language
Genes encoding ferredoxins from the transformable unicellular cyanobacterium Synechocystis sp. PCC 6803 and the thermophilic unicellular cyanobacterium Synechococcus vulcanus were cloned and sequenced. Southern blot hybridization detected a single copy of the ferredoxin sequences in both cyanobacterial genomes. Northern blot hybridization analyses indicated RNA transcripts of approximately 440 bases for Synechocystis sp. PCC 6803 and 460 bases for Synechococcus vulcanus . The accumulation of transcripts of the two cyanobacteria was three to four times greater in cells grown in iron‐rich medium than in cells grown in iron‐deficient medium.