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Ultraviolet‐B radiation causes shade‐type ultrastructural changes in Brassica napus
Author(s) -
Fagerberg Wayne R.,
Bornman Janet F.
Publication year - 1997
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1997.tb01071.x
Subject(s) - thylakoid , ultrastructure , chloroplast , photosynthetically active radiation , botany , biophysics , biology , brassica , palisade cell , sunlight , ultraviolet , irradiation , photosynthesis , biochemistry , optics , physics , gene , nuclear physics
Cell and chloroplast structural changes in palisade cells from mature leaves of Brassica napus L. cv. Paroll were quantified following exposure of plants to enhanced ultraviolet‐B (280–320 nm; 13 kJ m −2 day −1 biologically effective UV‐B) radiation at two different levels of photosynthetically active radiation (PAR, 400–700 nm; 200 and 700 μmol m −2 s −1 ). Short‐term changes in leaf ultrastructure after 30 min and longer term changes after one day and one week were analyzed using stereological techniques incorporating light and electron microscopy and mathematical reconstruction of a mean cell for each sample. Ultraviolet‐B together with either relatively high or low PAR resulted in cell structural changes resembling those typical of plants under shade conditions, with the most marked response occurring after 30 min of UV‐B radiation. The ultrastructural changes at the cellular level were generally similar in both the relatively high and low PAR plus UV‐B radiation treatments. The surface areas of all three thylakoid types, the appressed, non‐appressed and margin thylakoids increased in the palisade tissue under supplemental UV‐B irradiation. Although the appressed and non‐appressed thylakoids increased in surface area, they did not increase equally, leaving open the possibility that the two thylakoid types have independent regulatory systems or different sensitivity to UV‐B radiation. Increased thylakoid packing (mm 2 thylakoid membrane per mm 2 leaf surface) in UV‐B‐exposed plants may increase the statistical probability of photon interception. An increased level of UV‐absorbing pigments after one week of supplemental UV‐B radiation did not prevent or significantly ameliorate UV effects. Our data supported the assumption that UV‐B radiation may have a regulatory role besides damaging effects and that an increased UV‐B environment will likely increase this regulatory influence of UV‐B radiation.