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Expression of auxin‐inducible genes in relation to endogenous indoleacetic acid (IAA) levels in wild‐type and IAA‐overproducing transgenic tobacco plants
Author(s) -
Sitbon Folke,
Dargeviciute Ausra,
PerrotRechenmann Catherine
Publication year - 1996
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1996.tb06671.x
Subject(s) - auxin , biology , nicotiana tabacum , gene , endogeny , wild type , transgene , gene expression , complementary dna , microbiology and biotechnology , genetics , biochemistry , mutant
Probes corresponding to the auxin‐inducible genes parA and parB , as well as two aux genes, here denoted Nt‐aux8 and Nt‐aux16 , were amplified from tobacco ( Nicotiana tabacum L.) cDNA. Transcript levels of these genes were analysed in wild‐type tobacco plants and in the transgenic IAA‐overproducing line C, and related to the endogenous IAA level. In addition, effects of naphtyl‐1‐acetic acid (NAA) treatment on the expression of these genes were determined in both genotypes. Two separate situations were identified. Nt‐aux8 and Nt‐aux16 steady‐state mRNA levels were positively correlated with the IAA content in wild‐type and line C plants. Addition of NAA induced these two genes strongly in wild‐type plants, but only slightly in line C plants. On the other hand, mRNA levels of the parA and parB genes varied more with the organ analysed and its age than with the IAA level, and were induced by NAA treatment to a similar extent in the two genotypes. The results suggest that expression of the two Nt‐aux genes is closely related to the endogenous auxin level, whereas expression of the parA and parB genes to a greater extent is influenced by additional factors.