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Interactions between respiration and inorganic phosphate uptake in phosphate‐limited cells of Chlamydomonas reinhardtii
Author(s) -
Weger Harold G.
Publication year - 1996
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1996.tb00526.x
Subject(s) - chlamydomonas reinhardtii , phosphate , respiration , inorganic phosphate , chlamydomonas , chemistry , biophysics , environmental chemistry , botany , biochemistry , biology , gene , mutant
Phosphate addition to P‐limited cells of Chlamydomonas reinhardtii resulted in an immediate increase in the rate of respiratory O 2 consumption. The respiration rate continued to increase for several minutes after the addition of P 1 . Similar patterns of P 1 stimulation of respiratory O 2 consumption were observed in the presence of cyanide (cytochrome oxidase inhibitor) and propyl gallate (alternative oxidase inhibitor). Stimulation of O 2 consumption was accompanied by rapid changes in levels of glycolytic intermediates. These changes were consistent with activation of ATP‐dependent phosphofructokinase and pyruvate kinase. The adenylate pool exhibited only minor perturbations, P 1 , uptake resulted in extracellular acidification, which continued for several minutes after the exhaustion of added P 1 , whereas exhaustion of extracellular P 1 resulted in a rapid decline in the O 2 consumption rate. These results are consistent with control of respiration in P‐limited cells occurring largely at the level of glycolysis.