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Isolation and cDNA cloning of an Em‐like protein from mung bean ( Vigna radiata ) axes
Author(s) -
Manickam Ayyanar,
Damme Els J. M.,
Kalaiselvi Kandasamy,
Verhaert Peter,
Peumans Willy J.
Publication year - 1996
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1996.tb00512.x
Subject(s) - vigna , complementary dna , biology , cyanogen bromide , radiata , biochemistry , microbiology and biotechnology , homology (biology) , storage protein , peptide sequence , amino acid , gene , botany
Until now no ‘early‐methionine‐labelled’ (Em) proteins have been reported in the Fabaceae. To check whether a previously isolated low‐molecular mass albumin from dry mung bean embryonic axes possibly corresponded to an Em‐like protein, the protein was purified, sequenced and its cDNA clone isolated and characterized. N‐terminal sequencing of cyanogen bromide cleavage products of the protein revealed homology with previously described Em‐like proteins from other species. Analysis of cDNA clones encoding the mung bean Em protein revealed the presence of two classes of Em proteins and confirmed their homology to the previously characterized Em‐like proteins. In vivo labelling and northern blot analysis further demonstrated that the mung bean protein is synthesized during early germination of the axes and that abscisic acid (ABA) extends its synthesis. It appears, therefore, that legumes also contain maturation‐specific, ABA‐responsive Em‐like proteins.