Premium
Effect of sulphur nutrition on redistribution of sulphur in vegetative barley
Author(s) -
Adiputra I Gede K.,
Anderson J. W.
Publication year - 1995
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1995.tb05534.x
Subject(s) - sulfur , hordeum vulgare , nutrient , chemistry , hordeum , agronomy , horticulture , botany , poaceae , biology , organic chemistry
Barley plants ( Hordeum vulgare cv. Clipper) were grown in nutrient solution containing 25 μ M sulphate and pulsed for 48 h with [ 35 S]sulphate. The plants were then returned to unlabelled 25 μ M sulphate and analysed for sulphur and 35 S‐label at various times. The sulphur content of each leaf increased to a maximum as it attained full expansion and then decreased by ca 38–50% over the ensuing 30 days. Expanding leaves had the highest specific radioactivity after the pulse but this declined as the leaves expanded. The first leaf to emerge during the chase also had a high specific radioactivity. The data are consistent with the proposal that the sulphur required for early leaf development is derived from a previously formed leaf. 35 S‐Labelled sulphur which was delivered to fully expanded leaves did not equilibrate with endogenous sulphur and was selectively re‐exported. In another experiment, barley plants were grown in 5 μ M sulphate, pulsed for 48 h with [ 35 S]sulphate and then grown at 0 (S 0 ), 5 (S s ) or 25 μ M (S 25 ) unlabelled sulphate. In S 25 plants, the sulphur content of both the fully expanded leaves and the expanding leaves increased indicating that the control of sulphur allocation in these plants differed from that in plants grown continuously in 25 μ M sulphate and in plants which were grown at 0 or 5 μ M sulphate (sub‐optimal for the growth of leaves L7 and L8). The export of 35 S‐label from fully expanded leaves during the chase was not influenced by sulphur nutrition. However, the amount of 35 S‐label acquired by leaves which formed during the chase increased with sulphur nutrition. In S 25 plants these leaves subsequently underwent net loss of label but the analogous leaves of S n and S 5 plants did not. Collectively, the data indicate that, under sulphur limiting conditions, there is no evidence of a mechanism in barley which enhances the mobilization of sulphur from fully expanded leaves to young expanding leaves.