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Osmotin gene expression is controlled by elicitor synergism
Author(s) -
Chang PiFang Linda,
Cheah Kheng T.,
Narasimhan Meena L.,
Hasegawa Paul M.,
Bressan Ray A.
Publication year - 1995
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1995.tb05531.x
Subject(s) - elicitor , nicotiana tabacum , cellulase , biology , biochemistry , gene expression , pathogenesis related protein , reporter gene , plant defense against herbivory , microbiology and biotechnology , gene , enzyme
Arachidonic acid (AA), a fatty‐acid fungal elicitor, and a cellulase preparation from Aspergillus niger , a protein‐type fungal elicitor, induced osmotin gene expression. Both elicitors activated the osmotin promoter fused to a β‐glucuronidase (GUS) reporter gene in a tissue‐specific manner in tobacco seedlings ( Nicotiana tabacum L. cv. Wisconsin 38). The cellulase preparation was more effective than AA at the concentrations tested and, unlike AA, also induced the accumulation of osmotin mRNA and protein. Combinations of AA and the cellulase preparation had a greater than additive effect on the activation of the osmotin promoter and the accumulation of osmotin mRNA and protein. Both AA and the cellulase preparation, when applied separately, were virtually ineffective in the induction of the osmotin promoter in cotyledon tissues. However, together they were able to induce synergistically GUS fused to the osmotin promoter. Increases in osmotin‐promoter‐driven GUS activity and accumulation of osmotin mRNA induced by AA, the cellulase preparation or their combination were reversed by norbornadiene, an ethylene action inhibitor, indicating that ethylene is involved in the induction of the osmotin gene by these elicitors.