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Purification of intact chloroplasts from spruce ( Picea abies ) by free‐flow electrophoresis
Author(s) -
Afif Dany,
Canut Hervé,
Dizengremel Pierre
Publication year - 1995
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1995.tb05126.x
Subject(s) - chloroplast , picea abies , electrophoresis , population , botany , chemistry , chlorophyll , biology , chromatography , biochemistry , demography , sociology , gene
Highly purified chloroplasts were isolated from spruce ( Picea abies [L.] Karst.) needles by free‐flow electrophoresis. The chloroplast crude suspension was separated into two structurally different populations. The two populations could not be distinguished according to their protein/chlorophyll ratios and protein patterns from two‐dimensional gel electrophoresis. The O 2 evolution, however, showed differences: the chloroplast population deflected towards the cathode contained a major part of structurally intact chloroplasts in contrast to the population deflected towards the anode. The two populations were not contaminated by endoplasmatic membranes or mitochondria.