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Promotion by 2,4‐D of 7‐glucosylation of benzyladenine in seed‐derived and shoot apex‐derived cell cultures of Dianthus zeyheri
Author(s) -
Crouch N. R.,
Staden J.
Publication year - 1995
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1995.tb05112.x
Subject(s) - cytokinin , biology , callus , auxin , apex (geometry) , dianthus , botany , carnation , caryophyllaceae , shoot , explant culture , biochemistry , in vitro , gene
Cell suspension cultures derived from shoot apex or seed callus of the wild carnation Dianthus zeyheri were treated with [8 −14 ]benzyladenine. The metabolism of applied cytokinin was evaluated with time (30 min. 6 h. 18 h. 48 h) in the presence of both low (2 mg l −1 ) and high (4 mg 1 −1 ) levels of applied 2.4‐D. Both culture types metabolised BA to produce a qualitatively similar but quantitatively different array of metabolites. The 7‐glucoside of BA was the major metabolite produced by apex‐derived cultures, and the second largest product after the riboside in those cultures originating from seed. In both systems higher auxin application promoted 7‐glucosylation with a concurrent reduction in radioactivity associated with the‘active’ cytokinins. This suggests that cytokinin‐7‐glucosyl transferase is stimulated by the auxin 2.4‐D. and may, in part, explain the observed antagonistic interaction between these hormone classes.