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Analysis of leaf proteins in late flowering mutants of Arabidopsis thaliana
Author(s) -
Tacchini P.,
Fink A.,
Thiellement H.,
Greppin H.
Publication year - 1995
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1995.tb02234.x
Subject(s) - mutant , biology , locus (genetics) , arabidopsis thaliana , wild type , phenotype , botany , arabidopsis , genetics , horticulture , gene
Late flowering monogenic mutants of Arabidopsis thaliana (L.) Heynh. at the loci co, gi, fca, fve, fwa, fha, fpa, fy and their corresponding wild type, Landsberg erecta , were analysed by two‐dimensional gel electrophoresis. All plants were grown under continuous light and proteins were extracted from leaves of the same age (20‐day‐old). The polypeptide patterns of the mutants at the loci co, gi, fca, fve, fwa, fha, fpa , and Landsberg erecta were identical. The mutant at the fy locus showed a qualitative difference with Landsberg erecta . Crosses were made between this line and the wild type Landsberg erecta . F2 plants, resulting from autopollination of the hybrid, were analysed and showed no cosegregation between the observed protein and the flowering phenotype, indicating that these two lines differ by more than a single mutation.