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Multiple forms of nitrate reductase and their role in nitrate assimilation in roots of wheat at low temperature or high salinity
Author(s) -
Kenjebaeva Saule,
Rakova Natasha
Publication year - 1995
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1995.tb02224.x
Subject(s) - elution , nitrate reductase , chemistry , nitrate , salinity , enzyme , salt (chemistry) , nitrogen assimilation , reductase , chromatography , biochemistry , biology , organic chemistry , ecology
NADPH‐, NADH‐, and KNO 3 ‐eluted fractions of nitrate reductase (NR) were isolated from roots of winter wheat ( Triticum aestivum L. cv. Mironovskaya 808) grown under low temperature or high salinity. All three fractions exerted activity with either NADPH or NADH as electron donor. The NADPH‐eluted fraction showed the highest activity with NADPH, whereas the NADH‐ and KNO 3 ‐eluted fractions were most active with NADH. The NADH‐ and NADPH‐dependent activities in the NADH‐ and KNO 3 ‐eluted fractions were the ones that changed the most in response to low temperature. The inhibitory effect of salt stress was the same for both activities in each of the NADH‐ and KNO 3 ‐eluted fractions. The NADPH‐eluted NR was the one least affected by the growth conditions.