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Changes in the activity of 3‐deoxy‐D‐arabino‐heptulosonate 7‐phosphate (DAHP) synthase in suspension‐cultured cells of Vitis
Author(s) -
Suzuki Nao,
Sakuta Masaaki,
Shimizu Seki,
Komamine Atsushi
Publication year - 1995
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1995.tb00972.x
Subject(s) - biochemistry , shikimate pathway , biosynthesis , phenylalanine , phenylalanine ammonia lyase , enzyme , atp synthase , aromatic amino acids , enzyme assay , biology , amino acid , anthocyanin , phosphate , lyase , botany
3‐Deoxy‐D‐arabino‐heptulosonate 7‐phosphate (DAHP) synthase (EC 4.1.2.15) is the first enzyme in the shikimate pathway, which leads to the biosynthesis of the aromatic amino acids. These amino acids are utilized as precursors for the synthesis of some secondary metabolites. The relationship between the accumulation of anthocyanin and the activity of DAHP synthase in suspension cultures of Vitis hybrid (Bailey Alicante A) was investigated. The activity of the plastidic isozyme, designated DS‐Mn, was very low throughout the culture of cells. However, the activity of the cytosolic isozyme, designated DS‐Co, increased transiently and then decreased after transfer of cells to fresh medium, reaching minimum levels during the logarithmic phase. Thereafter, the activity of DS‐Co increased rapidly prior to the accumulation of anthocyanin. When phosphate was removed from the culture medium, growth of cells was limited and rapid accumulation of anthocyanin occurred, coincident with the termination of cell division. The activity of phenylalanine ammonia‐lyase continued to increase from day 1 and the activity of DS‐Co in phosphate‐free culture also was 1.6‐fold greater than that in the control culture on day 1, while the activity of DS‐Mn was unaffected by this treatment. These results suggest a close correlation between the activity of DS‐Co and the biosynthesis of anthocyanin.