Premium
The possible role of fructose 2,6‐bisphosphate in the cyanide‐mediated removal of embryonic dormancy in apple
Author(s) -
Bogatek Renata
Publication year - 1995
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1995.tb00954.x
Subject(s) - fructose 2,6 bisphosphate , phosphofructokinase , glycolysis , dormancy , fructose , cyanide , chemistry , enzyme , biochemistry , biology , germination , botany , inorganic chemistry
The effects of pretreatment with HCN on the level of fructose 2.6‐bisphosphate (F‐2,6‐P 2 ). on activity of fructose 6‐phosphate 2‐kinase (EC 2.7.1.150; F‐6‐P. 2K. enzyme synthesizing F‐2,6‐P 2 ), as well as on activities of PP 1 ‐dependent and ATP‐dependent phosphofructokinases (EC 2.7.1.90. PP 1 ‐PFK and EC 2.7.1.11, ATP‐PFK) were studied in cultured, dormant embryos of apple ( Malus domestica Borb. cv. Antonówka). HCN increased the F‐2.6‐P 2 level and F‐6‐P, 2K activity in embryonal axes (3‐fold), but had no effect in cotyledons. HCN pretreatment of embryos or the addition of F‐2.6‐P 2 to enzyme extract stimulated PP 1 , ‐PFK activity, whereas the activity of ATP‐PFK was slightly inhibited by HCN in axes and in cotyledons. Glycolysis is one of the first processes in the germination of apple embryos, and the stimulation of glycolysis by HCN may be the result of F‐6‐P, 2K activation in the axes. This will lead to accumulation of F‐2,6‐P 2 , which, in turn, enhances glycolysis by activation of PP 1 PKF.