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Phase reversal in Sequoia sempervirens in relation to mtDNA
Author(s) -
Huang LiChun,
Lin LihYuan,
Chen ChungMong,
Chen LiJeng,
Huang BauLian,
Murashige Toshio
Publication year - 1995
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1995.tb00942.x
Subject(s) - sequoia , juvenile , mitochondrial dna , biology , shoot , botany , rootstock , nuclear dna , ecori , dna , genetics , restriction enzyme , gene
Restriction fragment analysis revealed differences in mitochondrial DNA (mtDNA) between adult and juvenile coastal redwood, Sequoia sempervirens (D. Don) Endl. Thus, for example, juvenile shoots contained 4.0‐ and 3.6‐kb Bam HI], restricted fragments that were absent in adult material. Rejuvenation by 5 repetitive graftings onto juvenile rootstocks resulted in the same 4.0‐ and 3.6‐kb fragments as were present in the juvenile material. The similarities in mtDNA fragments of juvenile and rejuvenated. shoots, as well as their distinction from adult shoots, were confirmed by double digestion, Southern hybridization and DNA sequencing.