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Wounding regulates polysomal incorporation of hsp70 and tch1 transcripts during signal storage and retrieval
Author(s) -
HenryVian Chantal,
Vian Alain,
Davies Eric,
Ledoigt Gérard,
Desbiez MarieOdile
Publication year - 1995
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1995.tb00853.x
Subject(s) - polysome , biology , northern blot , hypocotyl , gene expression , rna , messenger rna , microbiology and biotechnology , permissive , wound healing , incubation , biochemistry , gene , ribosome , botany , genetics
In Bidens pilosa L. (var. radiatus ), wounding of both cotyledons induces the inhibition of hypocotyl growth. This morphogenic response occurs only if plantlets have been tranferred 24 h before from a non‐permissive (ion‐rich) to a permissive (deionized water) incubation medium. We have defined three kinds of experimental conditions where the wound‐response depends on the ionic content of the incubation medium: (1) permissive, allowing inhibition of hypocotyl growth; (2) non‐permissive, leading to storage of the wound signal; and (3) conditions required to recall the stored wound signal. Under these three experimental conditions, the accumulation of two stress‐related genes, tch1 (calmodulin) and hsp70 , was investigated using northern blots of poly(A) + and polysomal RNA. Under permissive conditions the wound response was characterized by a marked increase in tch1 and hsp70 ‐like poly(A) + RNA and by a slight association of these mRNAs with polysomes, 30 to 60 min after mechanical perturbation. In contrast, under non‐permissive conditions, we only observed a decrease in the accumulation of the two transcripts incorporated into polysomes. Under conditions that allow expression of the previously‐stored wound signal, we showed an increase in transcript accumulation ( hsp70 ‐like gene) or an increase in the level of mRNA associated with polysomes ( tch1 ‐like gene). These results suggest that transcriptional and post‐transcriptional regulation may occur at least at the level of transcript incorporation into the polysomes. This mechanism would be involved both after the immediate wound response and during the storage of the wound information.