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Effect of applied benzyladenine on endogenous cytokinin content during the early stages of bud development of kiwifruit
Author(s) -
Feito I.,
Rodríguez A.,
Centeno M. L.,
SánchezTamés R.,
Fernández B.
Publication year - 1995
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1995.tb00833.x
Subject(s) - explant culture , cytokinin , zeatin , endogeny , botany , biology , polyclonal antibodies , chemistry , biochemistry , in vitro , auxin , antibody , immunology , gene
A short‐term study linked directly to our previous long‐term experiments was conducted to clarify whether or not benzyladenine (BA) acts directly or through endogenous cytokinins in the development of kiwifruit explants. The uptake and metabolism of BA were characterized in kiwifruit explants after 0.5, 1, 2, 8 and 16 h exposure to 8‐[ 14 C]BA on both solidified and liquid Murashige and Skoog media. Endogenous cytokinins were measured by enzyme‐linked immunosorbent assay (ELISA) using polyclonal antibodies. The kinetics of BA uptake by explants cultured under these conditions were different, being higher in explants cultured in liquid medium. The glucosylation rate of BA was faster in explants cultured on solid medium. The levels of active forms of BA (BA and [9R]BA) were higher in explants cultured on liquid medium due to its higher uptake and lower glucosylation. The levels of zeatin‐type cytokinins were influenced by the culture conditions. A direct correlation was observed between morphogenic response and proportion of active forms of BA, but not with zeatin‐type cytokinins.