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Photoreduction of monovinyl‐ and divinyl‐protochlorophyllide in vitro and in vivo in the light‐dependent greening mutant C‐2A' of Scenedesmus obliquus
Author(s) -
Knaust Rosemarie,
Urbig Thomas,
Senger Horst
Publication year - 1995
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1995.tb00819.x
Subject(s) - protochlorophyllide , greening , mutant , oxidoreductase , in vivo , scenedesmus obliquus , biochemistry , in vitro , chlorophyll , endogeny , chemistry , biology , microbiology and biotechnology , enzyme , botany , algae , gene , genetics , ecology
Dark‐grown cells of mutant C‐2A' of Scenedesmus obliquus accumulate monovinyl‐and mainly divinyl‐protochlorophyllide (PChlide). Both PChlide‐forms are equally well photoconverted in vitro by the NADPH‐protochlorophyllide oxidoreductase of the light‐dependent greening mutant C‐2A’of Scenedesmus obliquus. The chlorophyllide (Chlide) resulting from this photoconversion in vivo has a predominantly monovinyl character. Only small traces of a transient Chlide‐form with divinyl fluorescence could be detected.