Premium
Biological activity, identification and quantification of gibberellins in seedlings of Norway spruce ( Picea abies ) grown under different photoperiods
Author(s) -
Moritz T.
Publication year - 1995
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1995.tb00809.x
Subject(s) - gibberellin , picea abies , photoperiodism , shoot , horticulture , botany , chemistry , biology
Short photoperiod induces growth cessation in seedlings of Norway spruce ( Picea abies (L.] Karst.). Application of different gibberellins (GAS) to seedlings growing under a short photoperiod show that GA 9 and GA 20 can not induce growth. In contrast application of GA, and GA 4 induced shoot elongation. The results indicate that 3β‐hydroxylation of GA 9 to GA 4 and of GA 20 to GA 1 is under photoperiodic control. To confirm that conclusion, both qualitative and quantitative analyses of endogenous GAs were performed. GA 1 , GA 3 , GA 4 , GA 7 , GA 9 , GA 12 , GA 15 , GA 15 , GA 20 , GA 29 , GA 34 and GA 51 were identified by combined gas chromatography‐mass spectrometry in shoots of Norway spruce seedlings. The effect of photoperiod on GA levels was determined by using deuterated and 14 C‐labelled GAs as intermal standards. In short days, the amounts of GA 9 , GA 4 and GA 1 are less than in plants grown in continuous light. There is no significant difference in the amounts of GA 3 , GA 12 , and GA 20 between the different photoperiods. The lack of accumulation of GA 9 and GA 20 under short days is discussed.