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Molecular cloning and characterization of vernalization‐related ( ver ) genes in winter wheat
Author(s) -
Chong Kang,
Wang LiPing,
Tan KeHui,
Huang HuaLiang,
Liang HouGuo
Publication year - 1994
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1994.tb08844.x
Subject(s) - vernalization , biology , complementary dna , gene , microbiology and biotechnology , cdna library , homology (biology) , plasmid , molecular cloning , cloning (programming) , recombinant dna , southern blot , clone (java method) , genetics , computer science , programming language
The enriched cold‐induced cDNA library of 1 × 10 4 plaque‐forming units (pfu. was prepared using cDNAs from cold‐induced winter wheat, subtracted with mRNA of the control (non cold‐induced). Results of the hybridization in situ of differential screening with probes of the control, the vernalized and devernalized wheat cDNAs showed that verc 17 ( verc : vernalization‐relatcd cDNA clone) is specific for the veinalization. The insert of lambda of 10 recombinant was subcloned into the sites between Bam HI and Hind III in a pUC 19 plasmid. Analysis by northern blotting with a probe from verc 17 indicated that the verc 17 has negative signals for the control and the devernatized mRNA, but a positive signal for the mRNA of vernalized wheat at about 1.8 kb. The sequence had 20 restriction sites, covered by 17 enzymes. The ver 17 gene showed some homology with the P. yeolii major merozoite surface‐antigen gene.