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Activities of plasma membrane‐bound enzymes isolated from roots of spruce (Picea abies) grown in the presence of aluminium
Author(s) -
Widell Susanne,
Asp Håkan,
Jensén Paul
Publication year - 1994
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1994.tb08836.x
Subject(s) - aluminium , membrane , chemistry , atpase , enzyme , picea abies , biochemistry , chromatography , biology , botany , organic chemistry
The effect of aluminium on plasma membrane‐bound enzymes and ion uptake was studied with spruce cuttings (Piccu abies Karst.) grown in nutrient solution, pH 4.2, for 5 weeks. The H + – ATPase and glucan synthase II activities of isolated root plasma membranes were inhibited by the presence of aluminium during growth (0.1–3 m M ), with glucan synthase II being more sensitive than the ATPase. The active K + ‐uptake obtained with intact roots was also inhibited by aluminium, whereas the passive uptake was stimulated ca 4‐fold by moderate amounts of aluminium (0.1 m M ). The uptake of Ca 2+ (passive) was severely inhibited at all external aluminium concentrations tested. Experiments were also performed with aluminium added during the assay, or in preincubations of isolated plasma membranes. Again, the glucan synthuse II activity was severely inhibited bv aluminium. There were no signs of adaptation, i.e., the activities with plasma membranes from cuttings given aluminium during growth, were equally sensitive as the control. The aluminium additions in vitro were at pH 6.5 (ATPase) or 7.2? (glucan synthase II) indicating that lor the enzymes studied, aluminium can be toxic although the pH is neutral.