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The protective effect of desferrioxamine on paraquat‐treated pea (Pisum sativum)
Author(s) -
Zer Hagit,
Peleg Itzhak,
Chevion Mordechai
Publication year - 1994
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1994.tb08833.x
Subject(s) - paraquat , chemistry , superoxide , chelation , lipid peroxidation , radical , pisum , biochemistry , glutathione reductase , glutathione , hydroxyl radical , antioxidant , enzyme , inorganic chemistry , glutathione peroxidase
Paraquat, a widely used herbicide, is photoreduced by photosystem I to the monovalent cation radical, which in turn, can react quickly and efficiently with molecular oxygen to produce superoxide anion radicals. In the presence of redox‐active iron (or copper) superoxide radicals can serve as a source for the more active species such as hydroxyl radicals. The present sludv investigated the possible mediatory role of iron in paraquat to xicity. The results demonstrate that desferrioxamme (0–150μM) a highiy specific iron chelator, reduces the loss of proteins (by 34–69%) and lipid peroxidation (by 31–96%) in paraquat treated leaf cuts. Dcsferrioxamine also protects malate dehydrogenase (61–70%) hydroxvpyruvate reductase (54–100%), and Ca 2+ ‐dependent ATPase (25–34%) against the paraquat‐induced loss of their activity. It also induces an increase in glutathione reductase activity (by 188%). These results, together with those from other experiments concerning the effect of desferrioxamine on paraquat uptake by the leaf cuts, suggest that the protection by desferrioxamine arises from its specific iron chelanon properties, and lead to the conclusion that nan‐protein‐bound and redoxactive forms of iron pluy a role in the manifestation of paraquat toxicity in plants.