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Identification and quantification of endogenous gibberellins in apical buds and the cambial region of Eucalyptus
Author(s) -
Hasan O.,
Ridoutt B.G.,
Ross J.J.,
Davies N.W.,
Reid J.B.
Publication year - 1994
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1994.tb08804.x
Subject(s) - gibberellin , chromatography , chemistry , eucalyptus globulus , endogeny , hydroxylation , botany , mass spectrometry , eucalyptus , biology , biochemistry , enzyme
Endogenous gibberellins (GAs) were extracted and purified from apical buds of Eucalyptus nitens (Deane and Maid.) Maid. and the cambial region of E. globulus (Labill.). then analysed by capillary gas chromatography‐mass spectrometry. GA 1 GA 19 GA 20 and GA 29 were identified by full scan mass spectra. Kovats retention indices and high resolution selected ion monitoring. Using deuterated internal standards. GA 1 . GA 19 . GA 20 and putative GA 29 and GA 53 were quantified in the apical buds, while GA 4 . GA 8 . GA 9 and GA 44 were shown to be either absent or present at very low levels. From the cambial region. GA 1 and GA 20 were quantified at levels of 0.30 ng (g fresh weight)‐ 1 and 8.8 ng (g fresh weight)‐ 1 respectively. These data suggest that the early 13‐hydroxylation pathway is the dominant pathway for GA biosynthesis in Eucalyptus .