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Endogenous hormone levels during direct somatic embryogenesis in Medicago falcata
Author(s) -
Ivanova Aneta,
Velcheva Margarita,
Denchev Plamen,
Atanassov Atanas,
Onckelen Henri A.
Publication year - 1994
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1994.tb06658.x
Subject(s) - somatic embryogenesis , zeatin , abscisic acid , biology , cytokinin , explant culture , embryogenesis , botany , embryo , hormone , endogeny , auxin , medicago , somatic cell , gibberellic acid , microbiology and biotechnology , in vitro , biochemistry , germination , gene
Endogenous indole‐3‐acetic acid, abscisic acid and cytokinins (zeatin, zeatin riboside, N‐isopentenyladenine and N‐isopentenyladenosine) were evaluated in initial explants (leaves) of in vitro propagated plants of alfalfa ( Medicago falcata L.) lines varying in embryogenic capacity and during the somatic embryogenesis process. Fast embryo‐genic induction was correlated with high IAA and low ABA levels in the initial explants. No significant differences were observed in the cytokinin contents. Our results suggest that a certain hormone balance is necessary to allow the expression of the embryogenic potential. The consistent stages of the direct somatic embryogenesis are also characterized by changes in hormonal levels.