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Actin in mating structures of Chlamydomonas eugametos gametes
Author(s) -
Kalshoven H.W.,
Brederoo J.,
Emons A.M.C.,
Musgrave A.,
Schuring F.,
Wells B.,
Ende H.
Publication year - 1994
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1994.tb03002.x
Subject(s) - biology , immunogold labelling , flagellum , mating , chlamydomonas , mating type , actin , microbiology and biotechnology , phalloidin , labelling , ultrastructure , botany , biochemistry , cytoskeleton , cell , zoology , gene , mutant
The presence of actin in Chlamydomonas eugametos mating structures was studied using monoclonal anti‐actin antibodies. Immunofluorescent labelling of mating gametes clearly stained their mating structures and this was confirmed at the electron microscope level by immunogold labelling of sections. Anti‐actin labelling also strongly stained the flagella at the flagellar collar regions and weakly stained the rest of the flagella. Treatment of gametes with 6–8% ethanol induced mating structures which protruded as large ‘balloons’. Balloons stained brilliantly with anti‐actin antibodies and weakly with FITC‐phalloidin, a fluorescent reagent that stains F‐actin. Isolated mating structure balloons and flagella were analyzed using western blotting. A prominent 43 kDa band, co‐migrating with actin in erythrocyte ghosts, reacted with anti‐actin antibodies. The results indicate that actin is present in mating structures and flagella of both mating types of C, eugametos .