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Abscisic acid: one of the factors affecting male sterility in Brassica napus
Author(s) -
Shukla Amit,
Sawhney V.K.
Publication year - 1994
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1994.tb02983.x
Subject(s) - abscisic acid , inflorescence , biology , sterility , brassica , stamen , botany , petal , pollen , horticulture , mutant , bud , biochemistry , gene
The possible involvement of abscisic acid (ABA) in the expression of male sterility in a genic male sterile (GMS) mutant of rapeseed ( Brassica napus ) was investigated. Three experimental approaches were used, namely, in vitro culture of young wild type (WT) floral buds, semi‐in vivo culture of WT inflorescences, and an analysis of endogenous ABA levels in various tissues of WT and GMS plants. Abscisic acid alone was inhibitory for the growth of WT floral buds in vitro, but low concentrations of ABA (10 −8 to 10 −6 M ) in combination with 10 −7 M benzyladenine, induced near‐mature flower bud development. With an increase in ABA concentration, the percentage of near‐mature floral buds decreased, and petal and stamen development was affected. Abscisic acid (10 −6 M and higher concentrations) also affected the flower bud length and pollen viability but not ovular viability, of WT inflorescences cultured in semi‐in vivo conditions. The various GMS tissues, including leaves, flowers and stamens had higher endogenous concentration of ABA in comparison to WT tissues. Also, the stamens had the maximum amount of ABA in comparison to other tissues in both genotypes. It is proposed that although ABA is important, it is only one of the plant growth substances involved in the expression of male sterility in the GMS line of B. napus .