Premium
Action spectra of phenylalanine ammonia‐lyase and chalcone synthase expression in carrot cells in suspension
Author(s) -
Takeda Junko,
Obi Ichiro,
Yoshida Kazuichi
Publication year - 1994
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1994.tb02982.x
Subject(s) - chalcone synthase , phenylalanine ammonia lyase , action spectrum , phenylalanine , chemistry , anthocyanin , biochemistry , biophysics , enzyme , biology , biosynthesis , photochemistry , food science , amino acid
The fluence‐response relationships and action spectra of light‐induced mRNA accumulation of phenylalanine ammonia‐lyase (PAL, EC 4.3.1.5) and chalcone synthase (CHS, EC 2.3.1.74) were investigated in light‐responsive, suspension‐cultured carrot cells after 2,4‐D‐depletion. The action spectrum for PAL mRNA accumulation (determined 6 h after a 1‐h light treatment) had a single peak around 290 nm and was similar to that of anthocyanin formation. The action spectrum for CHS mRNA accumulation, determined simultaneously, had two peaks, a sharp peak at 290 nm and a broad peak at 330 to 360 nm. Below 320 nm, the peak wavelength of the spectrum and the shape of the fluence‐response curve for CHS were similar to those of anthocyanin formation. The Bunsen‐Roscoe law of reciprocity in the response to UV‐B light was verified for PAL transcript accumulation, but not for that of CHS, suggesting that the system leading to the expression after UV‐B light perception was different between PAL and CHS.