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Trehalose and trehalase in root nodules from various legumes
Author(s) -
Müller Joachim,
Xie ZhiPing,
Staehelin Christian,
Mellor Robert B.,
Boller Thomas,
Wiemken Andres
Publication year - 1994
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1994.tb02196.x
Subject(s) - trehalase , trehalose , biochemistry , biology , root nodule , bradyrhizobium japonicum , rhizobium , legume , nitrogen fixation , disaccharide , bradyrhizobium , food science , bacteria , rhizobiaceae , botany , symbiosis , genetics , gene
Nitrogen‐fixing (effective) nodules from various legume‐ Rhizobium combinations were analyzed for trehalose and other soluble carbohydrates using gas chromatography and for trehalase activity using biochemical assays. Whereas the bacterial disaccharide trehalose was present only in the minority of the nodules, trehalase activity was found in all of them. Extracts from determinate nodules had a higher trehalase activity than extracts from indeterminate nodules. More detailed studies were done on soybean nodules formed in interactions with two effective and 5 ineffective Bradyrhizobium japonicum strains. Only in effective soybean nodules colonized by the strain 61‐A‐101 was trehalose a major soluble carbohydrate. Irrespective of the wildtype strains used. effective soybean nodules contained about 10 nkat trehalase g −1 fresh weight, whereas the ineffective nodules colonized by mutant strains derived from these wildtype strains contained 2 to 30 times less trehalase. However, a clear correlation between trehalose content and trehalase activity could not be established.