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Phosphatidylinositol metabolism in low temperature‐affected winter oilseed rape leaves
Author(s) -
SmoleńAskaSym Gabriela,
Kacperska Alina
Publication year - 1994
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1994.tb00651.x
Subject(s) - inositol , phosphatidylinositol , acclimatization , chemistry , brassica , phospholipase c , botany , horticulture , phosphate , metabolism , biology , biochemistry , enzyme , signal transduction , receptor
The aim of the present experiments was to find out whether low temperature may put into operation the phospholipase C‐inositolphospholipid signaling cascade in tissues of chilling‐tolerant plants. Therefore, the effects of a short and a rapid exposure of non‐acclimated (NA) and cold‐acclimated (CA) winter oilseed rape ( Brassica napus L. var. oleifera L. cv. Jantar) leaves to chilling (> 0°C) or freezing (‐5°C, ‐7°C) temperatures on the inositolphospholipid (PI, PIP and PIP 2 ) contents and [2‐ 3 H]‐inositol incorporation into phosphoinositides and inositol phosphates (IP 3 , IP 2 , and IP) were studied. LiCl (50 m M ) was used to arrest inositol phosphate hydrolysis on the IP level. Cold acclimation resulted in increased contents of PI and PIP 2 in the leaves. Rapid exposure of NA leaves to 0°C and CA leaves to ‐7°C (temperature of ice nucleation in the tissue) increased the amount of radioactivity in inositol phosphates and decreased the radioactivity in PIP 2 and PIP. The data indicate that low temperature induced phospholiphase C activity in winter rape leaves, the effectiveness of the treatment being dependent on the state of plant acclimation to cold. Mechanisms engaged and some biological implications of the responses observed for cold acclimation of plants are discussed.