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A Solanum (+) Potentilla interfamily protoplast regenerant with Solanum characteristics but with a Potentilla plastome fraction
Author(s) -
Wang Guirong,
Binding Horst
Publication year - 1994
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1994.tb00413.x
Subject(s) - protoplast , biology , chloroplast dna , plastid , potentilla , solanum , nuclear dna , dna , gene , microbiology and biotechnology , botany , genetics , genome , chloroplast , mitochondrial dna
A Potentilla ‐specific plastome fraction in a predominantly Solanum ‐type regenerant from a protoplast co‐culture was detected by RFLP. Fifty variegated lines from mixed protoplast populations of wildtype Potentilla caulescens and a Solanum hybrid containing albino‐mutant potato plastids were studied. The nuclear genome of all lines was uniparental Solanum , as indicated by morphology, isozyme patterns and chromosome number. Heterofamily plastid DNA was detected in one line by using 6 plastid DNA probes of barley. All of the Solanum restriction fragments were recovered. Additionally, probes of the psb A gene and 16Sr DNA revealed Potentllia ‐specific signals, and an ndh E+G gene probe detected a nonparental fragment. Only Solanum ‐type fragments hybridized with the psh A gene probe in albino sublines. Protoplast‐derived clones were either albinic or variegated. It is proposed that the line represents a cybrid with recombinant Solanum (+) Potentilla plastid DNA transmitted in combination with the entire Solanum plastid DNA.