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Effects of ethylene and auxin on polyamine levels in suspension‐cultured tobacco cells
Author(s) -
Park Ky Young,
Lee Sun Hi
Publication year - 1994
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1994.tb00403.x
Subject(s) - polyamine , auxin , spermine , spermidine , ornithine decarboxylase , ethylene , putrescine , nicotiana tabacum , arginine decarboxylase , biochemistry , chemistry , indole 3 acetic acid , biology , enzyme , catalysis , gene
The effects of ethylene and auxin on polyamine levels were studied in suspension‐cultured cells of tobacco ( Nicotiana tabacum . L). Treatment of 4‐day‐cultured cells with ethylene increased the levels of spermidine and spermine. The activities of arginine decarboxylase (ADC; EC 4.1.1.19), ornithine decarboxylase (ODC: EC 4.1.1.17), and S ‐adenosylmethionine decarboxylase (SAMDC: EC 4.1.1.50) rapidly increased between 3 and 12 h. An auxin, indole‐3‐acetic acid (IAA), increased polyamine levels and activities of ADC, ODC and SAMDC. The spermine level continued to increase significantly during a 24‐h incubation with IAA. The increases in polyamine accumulation induced by ethylene were partially offset by an inhibitor of ethylene action, 2,5‐norbornadiene. It is suggested that the auxin‐induced polyamine accumulation occurred directly, without metabolic competition between ethylene and polyamine biosynthesis, and indirectly, through auxin‐induced ethylene formation.