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Cellular responses of two rapid‐cycling Brassica species, B. napus and B. carinata , to seawater salinity
Author(s) -
He Tie,
Cramer Grant R.
Publication year - 1993
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1993.tb08790.x
Subject(s) - salinity , seawater , brassica carinata , brassica , artificial seawater , botany , biology , sodium , horticulture , chemistry , zoology , ecology , organic chemistry
Cellular responses of two rapid‐cycling Brassica species. B. napus and B. carinata , to seawater salinity were characterized to determine whether callus showed a tolerance similar to that of whole plants. Callus was initiated from the leaves of 7‐day‐old seedlings of B. napus and B . carinata and then subcultured with two different levels of seawater salinity (2.3 and 5.2 g l −1 Instant Ocean. Aquarium Systems, Inc. Mentor. OH, USA) for 14 days. Callus growth of both species was reduced by seawater salinity. Based on the percentage of the reduction in the relative fresh weight gain. B. napus was more salt‐tolerant than B. carinata . consistent with the response of whole plants of the same species to seawater salinity. Seawater salinity caused changes in the concentrations of Na, K. Ca, Mg and Cl in both B. napus and B. carinata . The growth expressed as the percentage of control was significantly (P = 0.05) positively correlated with the concentration of Ca. and K/Na and Ca/Na ratios. It was also negatively correlated ( P = 0.01) with the concentrations of Na. Cl and Mg. In comparison with B. carinala . the salt‐tolerant species, B . napus , showed a small reduction in the concentrations of Ca and K in the salt‐stressed plants relative to the control.