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Differential expression of DAHP synthase and chorismate mutase isozymes during rhizogenesis of Brassica juncea cultured in vitro
Author(s) -
Sharma Ranjana,
Bhatnagar Raj K.,
Sarin Neera Bhalla
Publication year - 1993
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1993.tb05500.x
Subject(s) - callus , chorismate mutase , isozyme , biology , shikimate pathway , biochemistry , atp synthase , brassica , enzyme , botany , biosynthesis
The regulatory patterns of two of the enzymes of the shikimate pathway. 3‐deoxy‐D‐arabino‐heptulosonate‐7‐phosphate synthase (DAHP synthase or DS. EC 4. 1. 2. 15) and chorismate motase (CM, EC 5. 4. 99. 5), were investigated using in vitro cultures of Brassica juncea at two stages, viz. undifferentiated, proliferating callus and the root‐forming callus. Our studies revealed the presence of the two isozymes of DAHP synthase, DS‐Mn and DS‐Co. in undifferentiated callus. However, during the rhizogenesis of the callus DS‐Mn was absent. Similarly, for chorismate mutase, whereas both the isozymes CM‐1 and CM‐2 were present in undifferentiated callus only CM‐2 was detected at rhizogenesis. The possible involvement of these isozymes in callus growth and rhizogenesis is discussed.