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Immunolocalization of a thiol‐protease induced during the senescence of unpollinated pea ovaries
Author(s) -
Cercós Manuel,
Harris Nick,
Carbonell Juan
Publication year - 1993
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1993.tb05499.x
Subject(s) - vacuole , senescence , pisum , biology , protease , sativum , ultrastructure , biochemistry , subcellular localization , microbiology and biotechnology , cytoplasm , enzyme , botany
A thiol‐endoprotease induced during the senescence of unpollinated ovaries of Pisum sativum L. cv. Alaska has been localized at both cellular and subcellular levels using purified antibodies. Immunoblot analysis showed a single band of 30 kDa in extracts from senescent ovaries 3 and 4 days post‐anthesis. Immunolocalization showed the accumulation of the protease within the exocarp and in the outer cell layers of the mesocarp of the senescent ovaries, although with an asymmetric distribution as illustrated in transverse sections. Ultrastructural localization indicates that the protease is associated with the tonoplast and with electron dense materials within the vacuole, where lysis of cell components occurs in senescent ovaries.