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Genetic basis of sodium exclusion and sodium tolerance in yeast. A model for plants
Author(s) -
Haro Rosario,
Bañuelos María A.,
Quintero Francisco J.,
Rubio Francisco,
RodríguezNavarro Alonso
Publication year - 1993
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1993.tb05298.x
Subject(s) - efflux , yeast , vacuole , sodium , transformation (genetics) , mutant , strain (injury) , biology , biochemistry , chemistry , microbiology and biotechnology , gene , organic chemistry , anatomy , cytoplasm
A yeast strain carrying disruptions in TRK1 and ENA genes was very sensitive to Na + because uptake discriminated poorly between K + and Na + , and Na + efflux was insignificant. Transformation with TRK1 and ENA1 restored discrimination, Na + efflux and Na + tolerance. Increasing external Ca 2+ increased Na + tolerance almost in the same proportion in TRK1 enal cells and in trkl ENAI cells, suggesting an unspecific effect of this cation. By using a vacuolar ATPase mutant, the role of the vacuole in Na + tolerance was also demonstrated. The yeast model of Na + exclusion and Na + tolerance may be extended to plants.