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Abscisic acid and gibberellic acid‐regulated responses of embryos and aleurone layers isolated from dormant and nondormant barley grains
Author(s) -
Beckum Joke M. M.,
Libbenga Kees R.,
Wang Mei
Publication year - 1993
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1993.tb05202.x
Subject(s) - aleurone , abscisic acid , germination , biology , dormancy , gibberellic acid , embryo , hordeum vulgare , botany , rab , hordeum , gibberellin , microbiology and biotechnology , endosperm , biochemistry , poaceae , gene , gtpase
Dormant and nondormant isogenic barley grains were obtained by maturing grains under short day (SD) or long day (LD) growth conditions, respectively. Hormonal responses of isolated embryos and aleurone layers from these grains were studied. Addition of abscisic acid (ABA) reduced germination rate and percentage of embryos, and induced Rab (ABA‐responsive) mRNA in aleurone layers from both types of grain. Embryos and aleurone layers from dormant grains responded stronger to ABA than those from nondormant grains. Gibberellic acid (GA 3 ) increased the germination rate and percentage of embryos from dormant grains and counteracted the ABA‐induced inhibition of embryo germination. GA 3 did not affect the amount of Rab mRNA in aleurone layers, suggesting that expression of the Rab gene has no direct correlation with germination. The stronger response of embryos and aleurone layers from dormant grains to ABA may not be explained by higher endogenous ABA levels, but might be due to differences in hormone signal transduction. Aleurone protoplasts from dormant grains had a higher cytosolic pH than those from nondormant grains. To inhibit the ABA‐induced Rab mRNA, a much higher concentration of weak acid was required for aleurone layers from dormant grains than for those from nondormant grains. A possible difference in ABA signal transduction between dormant and nondormant grains is discussed.