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Iron stress‐induced redox reactions in bean roots
Author(s) -
Schmidt Wolfgang
Publication year - 1993
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1993.tb05197.x
Subject(s) - ferricyanide , redox , vanadate , phaseolus , chemistry , atpase , electron transport chain , biochemistry , biophysics , enzyme , inorganic chemistry , botany , biology
Iron stress‐induced and constitutive redox activity of bean ( Phaseolus vulgaris L. cv. Delinel) roots was measured on intact plants using FeEDTA and ferricyanide as electron acceptors. The presence of the translation inhibitor cycloheximide caused a decrease in the reduction of both oxidants. However, a differential decline in the reduction rates of FeEDTA and ferricyanide was observed, suggesting enzyme heterogeneity. In the presence of the H + ‐ATPase inhibitor vanadate, the reduction of FeEDTA was nearly completely suppressed in both Fe‐deficient (–Fe) and Fe‐sufficient (+Fe) plants, providing evidence for an involvement of plasma membrane‐bound ATPase activity in the regulation of the reduction process. The inhibition of the ferricyanide reduction by vanadate was restricted to –Fe plants. The data are interpreted in terms of simultaneous operation of distinct redox systems in roots of iron‐deficient bean plants. The role of proton extrusion in iron stress‐induced electron transfer is discussed.