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Benzyladenine‐induced changes in the translatable mRNA population in excised cucumber cotyledons
Author(s) -
Teramoto Haruhiko,
Momotani Eiki,
Tsuji Hideo
Publication year - 1993
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1993.tb02510.x
Subject(s) - isoelectric focusing , polyacrylamide gel electrophoresis , gel electrophoresis , electrophoresis , population , messenger rna , biology , rna , microbiology and biotechnology , chemistry , biochemistry , gene , enzyme , demography , sociology
Benzyladenine‐induced changes in the translatable mRNA population in excised cucumber cotyledons were studied. Poly (A) + RNA was prepared from etiolated cotyledons incubated with or without benzyladenine (BA) for various periods in the dark. Using nonequilibrium pH gradient electrophoresis‐SDS polyacrylamide gel electrophoresis and isoelectric focusing‐SDS polyacrylamide gel electrophoresis, both basic and neutral proteins translated in vitro were separated. About 240 spots were detected and 16 of them changed within 6 h after BA application. Some spots changed quickly (within 1–2 h). Among them, three were repressed markedly