Premium
Evidence for phosphorylation of tubulin in carrot suspension cells
Author(s) -
Koontz Deborah A.,
Choi Jung H.
Publication year - 1993
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1993.tb02509.x
Subject(s) - daucus carota , tubulin , phosphoprotein , phosphorylation , polyclonal antibodies , antiserum , biology , biochemistry , adenosine triphosphate , in vitro , in vivo , immunoprecipitation , microbiology and biotechnology , microtubule , botany , antibody , gene , immunology
Two‐dimensional gels of phosphoproteins from carrot ( Daucus carota L. var. Juwarot) suspension cells labeled in vivo or in vitro revealed phosphoproteins that comigrate with carrot tubulin. A polyclonal antiserum to hibiscus tubulin immunoprecipitated an in vivo labeled phosphoprotein of 50 kDa. Cell‐free extracts of carrot suspension cells phosphorylated both purified carrot and bovine brain tubulins in the presence of gamma‐labeled adenosine triphosphate. This tubulin phosphorylating activity was reduced 2‐fold in extracts from globular stage embryos and approximately 10‐fold in extracts from heart/torpedo stage embryos. These data suggest that carrot cells phosphorylate tubulin, and that tubulin phosphorylating activity may be developmentally regulated