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Effects of triadimefon and osmotic stress on plasma membrane composition and ATPase activity in white spruce ( Picea glauca ) needles
Author(s) -
Sailerova Eva,
Zwiazek Janusz J.
Publication year - 1993
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1993.tb02496.x
Subject(s) - triadimefon , osmotic shock , chemistry , phospholipid , atpase , membrane , sterol , biochemistry , osmoregulation , chromatography , botany , biology , enzyme , cholesterol , ecology , salinity , fungicide , gene
White spruce [ Picea glauca (Moench) Voss.] seedlings were grown in solution culture and treated with 20 mg I ‐1 triadimefon [1‐(chlorophenoxy)‐3,3‐dimethyl‐1‐(1,2,4‐triazol‐1‐yl)‐2‐butanol] for 4 weeks and then subjected to osmotic stress with polyethylene glycol 3350. Water potentials and electrolyte leakage were measured in control and triadimefon‐treated seedlings before and after the plants were subjected to osmotic stress. The plasma membranes were isolated from needles to study their lipid composition and the activity of plasma‐membrane bound ATPase. Triadimefon treatment reduced water potentials and increased leakage of electrolytes from seedlings. However, when the seedlings were exposed to osmotic stress, triadimefon‐treated plants maintained higher water potentials and leaked less electrolytes compared with the control plants. Both triadimefon and osmotic stress treatments inhibited the activity of plasma membrane‐bound ATPase and altered the composition of free sterols in the plasma membranes. Triadimefon‐treated plants contained traces of campesterol, which was not present in control. Osmotic stress drastically reduced phospholipid:protein and sterol:protein ratios and increased sterol:phospholipid ratios in the plasma membranes