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Cold‐induced changes in the polysome pattern and protein synthesis in winter rye ( Secale cereale ) leaves
Author(s) -
Antikainen Mervi,
Pihakaski Seppo
Publication year - 1993
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1993.tb01793.x
Subject(s) - secale , polysome , cold stress , biology , methionine , greening , horticulture , botany , caryopsis , biochemistry , poaceae , ribosome , rna , amino acid , ecology , gene
Cold‐induced changes in the polysome pattern and protein synthesis were analyzed in winter rye, Secale cereale L. cv. Voima, during one week's cold stress treatment, which was performed by transferring the 7‐day‐old plants from the greenhouse (25°C, long‐day conditions) to 3°C and a photoperiod of 10. 5 h. Freezing resistance determined by electrolyte leakage increased significantly upon cold stress starting from LT 50 value –5°C. and reaching –9°C on the day 7 of cold exposure. After 4 weeks at low temperature, plants reached an LT 50 of –12°C. The polysome content increased markedly during cold stress compared to the control plants. After 2 weeks of cold treatment the polysome content decreased to the same level as that in control plants. The size‐class distribution of polysomes showed a high proportion of large protein synthesizing polysomes in cold‐stressed plants. After 2 weeks the values were comparable to those in control plants. Cold‐induced proteins were detected using 35 S‐labelled methionine for in vitro translations. At least 2 new polypeptides, M r 30000 and 18000, were induced on the first day of cold stress and continued to be expressed at low temperatures 4 weeks later.