Premium
Elicitation of H 2 O 2 production in cucumber hypocotyl segments by oligo‐1,4‐α‐D‐galacturonides and an oligo‐β‐glucan preparation from cell walls of Phythophthora megasperma f. sp. glycinea
Author(s) -
Svalheim Øystein,
Robertsen Børre
Publication year - 1993
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1993.tb01388.x
Subject(s) - phytophthora megasperma , cucumis , hypocotyl , elicitor , glucan , cell wall , biochemistry , oligosaccharide , polysaccharide , chemistry , biology , botany , enzyme
The production of H 2 O 2 by cucumber hypocotyl segments ( Cucumis sativus L. cv. Wisconsin SMR 58) in response to α‐1,4‐linked oligomers of galacturonic acid and oligo‐β‐glucans from the cell walls of Phytophthora megasperma f. sp. glycinea was studied. Oligogalacturonides with degrees of polymerization of 9 to 13 elicited H 2 O 2 production, the most effective being the deca‐, undeca‐ and dodecamers. A similar relationship between size and effect was previously obtained when oligogalacturonides were tested for their ability to elicit lignification in cucumber hypocotyls. The oligogalacturonide‐induced increase in H 2 O 2 concentration was detected after 4 h, reaching a maximum after 10 h of incubation. The glucan elicitor induced lignification at a 100‐fold lower concentration than the oligogalacturonides, but yielded only 10% of the maximum H 2 O 2 accumulation seen with oligogalacturonides. The glucan elicitor‐induced H 2 O 2 production was detectable after 2 h, and reached a maximum after 4 to 6 h. Catalase abolished the elicitation of both phenol red oxidation and lignification in cucumber hypocotyls. At least part of the oligogalacturonide‐induced H 2 O 2 production appeared to be dependent upon de novo protein synthesis.