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T L ‐DNA transformation decreases ABA level
Author(s) -
Julliard Jacques,
Pelèse Florence,
Sotta Bruno,
Maldiney Régis,
PrimardBrisset Catherine,
Jouanin Lise,
Pelletier Georges,
Miginiac Emile
Publication year - 1993
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1993.tb01385.x
Subject(s) - brassica , nicotiana tabacum , biology , brassica oleracea , transformation (genetics) , botany , agrobacterium , horticulture , gene , biochemistry
The endogenous levels of ABA were measured in Agrobacterium rhizogenes A 4 T l ‐DNA transformed oilseed rape (Brassica napus L. var. oleifera cv . Brutor and cv. Drakkar), cabbage (Brassica oleracea) . A 4 transformed tobacco ( Nicotiana tabacum cv. Xanthi) and their normal counterparts, using high performance liquid chromatography and enzyme‐liked immunosorbent assay. Measurements were made on different plant tissues (i. e. floral stem, terminal bud, young leaf, mature leaf, root and root tips) and ABA levels were compared in unstressed and osmotically stressed oilseed rape plants (cv. Brutor). In unstressed Plants. in each of the 5 independent transformation events studied, a significant reduction (about 65% of control) in ABA concentration was observed in all transformed plants. When subjected to an osmotic stress, T L transformed Brutor plants showed a higher ABA accumulation than untransformed plants. The change in ABA content as a consequence of T L ‐DNA transformation is discussed with regard to phenotype, drought resistance and adaptability.