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Biochemical and physiological responses of Gracilaria tenuistipitata uuder two different nitrogen treatments
Author(s) -
GarcíaSánchez María J.,
Fernández José A.,
Niell F. X.
Publication year - 1993
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1993.tb01382.x
Subject(s) - rubisco , phycobiliprotein , chemistry , food science , nitrate , nitrogen , botany , pigment , phycoerythrin , photosynthesis , horticulture , biology , biochemistry , zoology , cyanobacteria , microbiology and biotechnology , bacteria , flow cytometry , organic chemistry , genetics
The influence of nitrogen on ribulose‐1.5‐bisphosphate carboxylase/oxygenase (Rubisco. EC 4.1.1.39) content is poorly understood in macroalgae. N‐deficient Gracilaria tenuistipitata Zhang et Xia var. liui was cultivated in the laboratory under constant light intensity and temperature. Biochemical and physiological variables were monitored after a high (1 m M ) or low (o. 1 m M ) nitrate pulse. Rubisco content in crude extracts was estimated by SDS‐PAGE with the Coomassie Blue Staining procedure. Nitrate was consumed immediately in the low‐N treatment, but there was always an external nitrate source in the high‐N treatment. Total soluble proteins and phycobiliproteins decreased as internal nitrogen diminished in the low‐N treatment, but kept fairly constant in N‐sufficient conditions. However, Rubisco content increased until the 7th day and then started to decrease in both cases. Fresh weight increment showed a better correlation with Rubisco than with pigment content.