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Immuno‐gold localization of IAA in leaf cells of Prunus persica at different stages of development
Author(s) -
Ohmiya Akemi,
Hayashi Tateki
Publication year - 1992
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1992.tb05809.x
Subject(s) - prunus , chloroplast , indole 3 acetic acid , colloidal gold , parenchyma , cytosol , botany , chemistry , biology , biochemistry , microbiology and biotechnology , enzyme , auxin , materials science , nanotechnology , nanoparticle , gene
Subcellular localization of indole‐3‐acetic acid (IAA) in leaf cells of peach ( Prunus persica [L.] Batsch ‘Hakuho’) was investigated using imuno‐gold electron microscopy. The distribution pattern of the gold particles, which detected IAA, changed as cells matured. The most prominent feature was the accumulation of the gold label in the chloroplasts and mitochondria of the parenchyma cells of opened leaves. Throughout the development, the cytosol, nuclei, and cell wall were labelled, although the level was low and no significant changes occurred. The density of colloidal gold at each stage of leaf development was well correlated with the analytical data obtained by high‐performance liquid chromatography (HPLC).