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Changes with time in the H + concentration of the culture medium influences morphogenesis in tobacco thin cell layers
Author(s) -
Cousson Alain,
Tran Thanh Van Kiem,
Trinh Toan Hanh
Publication year - 1992
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1992.tb05270.x
Subject(s) - kinetin , morphogenesis , callus , organogenesis , explant culture , biology , botany , nicotiana tabacum , shoot , butyric acid , auxin , tissue culture , vegetative reproduction , biochemistry , in vitro , gene
Different types of morphogenesis in thin cell layers of Nicotiana tabacum cv. Samsun were studied in relation to changes in the external H + concentration during cluture. Different initial pHs of the medium, ranging from 3.83 to 6.35, were tested under unbuffered and MES‐buffered conditions, in combination with various amounts of indolyl‐3‐butyric acid and kinetin. The explants were sequentially transferred from MES‐free media to MES‐supplemented media, as well as reciprocally, to determine possible periods during the morphogenic process that showed a particular sensitivity to the external pH. Starting from pH 3.83, 36 m M MES induced the formation of limited callus and of vegetative and floral shoots as flowers in the control. MES at 50 m M inhibited rhizogenesis and either prevented morphogenesis or induced vegetative buds or flowers, depending on the initial pH. The 4th day of culture was a determining period in the induction of roots and flowers. Rhizogenesis, but not floral or vegetative organogenesis, was related to the theoretical intracellular concentration of indolyl‐3‐butyric acid. H + transport might be involved in the regulation of morphogenesis.