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Plant cell wall removal: Cause for microtubule instability and division abnormalities in protoplast cultures?
Author(s) -
Simmonds Daina H.
Publication year - 1992
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1992.tb04751.x
Subject(s) - protoplast , microtubule , cell wall , mitosis , cell division , biology , cell , microbiology and biotechnology , plant cell , cell culture , transmembrane protein , biophysics , botany , biochemistry , genetics , gene , receptor
Cell wall removal from plant cells can destabilize the cortical microtubules (MTs) in isolated protoplasts. The degree of destabilization depends on the origin and physiological condition of the cells, enzyme purity and digestion protocol, and the presence, in the digestion medium, of stabilizing factors such as Ca 2+ or taxol. Disorientation of MTs in protoplasts and the absence of a “normal’ cell wall during early culture periods results in abnormalities in mitotic spindles, phragmoplasts, new cross‐walls and chromosome segregation. These abnormalities are greatly reduced in older protoplast cultures, where a substantial cell wall had regenerated. It is suggested that the cell wall may serve to stabilize MTs through transmembrane proteins and may play a role in the spatial organization of MT nucleating sites.